Trypsin Autolysis Products
The trypsin used was side-chain protected porcine trypsin from Promega. The enzyme was derivatized by reductive methylation (Lys side-chains) to prevent autolysis. The data suggest that it underwent some autolysis prior to and/or during the derivatization. The peptides in bold were identified by LC/MS/MS experiments.
The Cys residues participate in disulfide-linkages. The list contains only N-terminally methylated or doubly methylated or Lys side-chain Me-derivatives.
- * N-methyl derivative
- ** N,N-dimethyl derivative
- C* half cystine
- Met(O) Met-sulfoxide
- sequence in bold - observed in digests and I'm pretty sure about the structure
- sequence in italics - observed in digests, identified by mass only
| m/z (mi) |
[Start-End] | Peptide Sequence |
|---|---|---|
| 451.1941 | [76-79] | (N)FNGN(T) |
| 462.2200 | [80-83] | (N)TLDN(D) |
| 515.3306 | [46-49] | (R)IQVR(L) |
| 569.2684 | [50-54] | (R)LGEHN(I) |
| 633.2844 | [78-83] | (N)GNTLDN(D) |
| 650.3150 | [62-66] | (N)EQFIN(A) |
| 664.3670 | [203-208] | (K)PGVYTK(V) |
| 694.39 | [70-75] | (K)IITHPN(F) |
| 711.3136 | [209-214] | (K)VCNYVN(W) |
| 732.4330 | [84-89] | (N)DIMLIK(L) |
| 748.4279 | [84-89] | (N)DIM(O)LIK(L) |
| 759.3889 | [55-61] | (N)IDVLEGN(E) |
| 792.4620 | [202-208] | (N)KPGVYTK(V) |
| 802.4311 | [90-97] | (K)LSSPATLN(S) |
| 820.4933 | [202-208] | (N)K**PGVYTK(V) |
| 842.5100 | [100-107] | (R)VATVSLPR(S) |
| 870.5412 | [100-107] | (R)V**ATVSLPR(S) |
| 906.5049 | [201-208] | (K)NKPGVYTK(V) |
| 920.4842 | [62-69] | (N)EQFINAAK(I) |
| 934.5362 | [201-208] | (K)NK**PGVYTK(V) |
| 955.5001 | [70-77] | (K)IITHPNFN(G) |
| 964.5580 | [67-75] | (N)AAKIITHPN(F) |
| 968.4511 | [1-10] | (-)IVGGYTCAAN(S) |
| 980.4875 | [203-211] | (K)PGVYTKVCN(Y) |
| 1006.4879 | [140-149] | (K)APVLSDSSCK(S) |
| 1006.5209 | [11-19] | (N)SVPYQVSLN(S) |
| 1008.5188 | [203-211] | (K)PGVYTK**VCN(Y) |
| 1023.4747 | [150-159] | (K)SSYPGQITGN(M) |
| 1044.5478 | [215-223] | (N)WIQQTIAAN(<) |
| 1045.5642 | [90-99] | (K)LSSPATLNSR(V) |
| 1126.5645 | [70-79] | (K)IITHPNFNGN(T) |
| 1153.5750 | [160-170] | (N)MICVGFLEGGK(D) |
| 1169.5699 | [160-170] | (N)M(O)ICVGFLEGGK(D) |
| 1175.6346 | [80-89] | (N)TLDNDIMLIK(L) |
| 1178.5264 | [20-31] | (N)SGSHFCGGSLIN(S) |
| 1191.6295 | [80-89] | (N)TLDNDIM(O)LIK(L) |
| 1309.6388 | [50-61] | (R)LGEHNIDVLEGN(E) |
| 1335.4946 | [171-184] | (K)DSC*QGDSGGPVVC*N(G) |
| 1337.5102 | [171-184] | (K)DSCQGDSGGPVVCN(G) |
| 1378.6578 | [32-43] | (N)SQWVVSAAHCYK(S) |
| 1390.6854 | [55-66] | (N)IDVLEGNEQFIN(A) |
| 1420.7225 | [212-223] | (N)YVNWIQQTIAAN(<) |
| 1469.7310 | [126-139] | (K)SSGSSYPSLLQCLK(A) |
| 1515.8456 | [84-97] | (N)DIMLIKLSSPATLN(S) |
| 1531.8405 | [84-97] | (N)DIM(O)LIKLSSPATLN(S) |
| 1537.6416 | [108-123] | (R)SC*AAAGTEC*LISGWGN(T) |
| 1539.6572 | [108-123] | (R)SCAAAGTECLISGWGN(T) |
| 1543.8768 | [84-97] | (N)DIMLIK**LSSPATLN(S) |
| 1559.8718 | [84-97] | (N)DIM(O)LIK**LSSPATLN(S) |
| 1621.7909 | [32-45] | (N)SQWVVSAAHCYKSR(I) |
| 1649.8221 | [32-45] | (N)SQWVVSAAHCYK**SR(I) |
| 1694.8325 | [185-200] | (N)GQLQGIVSWGYGCAQK(N) |
| 1698.8737 | [124-139] | (N)TKSSGSSYPSLLQCLK(A) |
| 1726.9049 | [124-139] | (N)TK**SSGSSYPSLLQCLK(A) |
| 1736.8430 | [209-223] | (K)VCNYVNWIQQTIAAN(<) |
| 1766.7842 | [108-125] | (R)SC*AAAGTEC*LISGWGNTK(S) |
| 1768.7998 | [108-125] | (R)SCAAAGTECLISGWGNTK(S) |
| 1808.8754 | [185-201] | (N)GQLQGIVSWGYGCAQKN(K) |
| 1836.9066 | [185-201] | (N)GQLQGIVSWGYGCAQK**N(K) |
| 1940.9354 | [50-66] | (R)LGEHNIDVLEGNEQFIN(A) |
| 1955.9537 | [1-19] | (-)IVGGYTCAANSVPYQVSLN(S) |
| 1959.0473 | [80-97] | (N)TLDNDIMLIKLSSPATLN(S) |
| 1975.0422 | [80-97] | (N)TLDNDIM(O)LIKLSSPATLN(S) |
| 1987.0785 | [80-97] | (N)TLDNDIMLIK**LSSPATLN(S) |
| 2003.0734 | [80-97] | TLDNDIM(O)LIK**LSSPATLN |
| 2010.9443 | [140-159] | (K)APVLSDSSCKSSYPGQITGN(M) |
| 2038.9755 | [140-159] | (K)APVLSDSSCK**SSYPGQITGN(M) |
| 2158.0313 | [150-170] | (K)SSYPGQITGNMICVGFLEGGK(D) |
| 2166.0290 | [11-31] | (N)SVPYQVSLNSGSHFCGGSLIN(S) |
| 2174.0262 | [150-170] | (K)SSYPGQITGNM(O)ICVGFLEGGK(D) |
| 2211.1046 | [50-69] | (R)LGEHNIDVLEGNEQFINAAK(I) |
| 2225.1202 | [50-69] | (R)LGEHNIDVLEGNEQFINAAK*(I) |
| 2239.1358 | [50-69] | (R)LGEHNIDVLEGNEQFINAAK**(I) |
| 2283.1807 | [70-89] | (K)IITHPNFNGNTLDNDIMLIK(L) |
| 2299.1756 | [70-89] | (K)IITHPNFNGNTLDNDIM(O)LIK(L) |
| 2435.1587 | (-)IVG*GYTCAAN(S) | |
| (K)SSGSSYPSLLQC*LK(A) | ||
| 2455.1849 | [126-149] | (K)SSGSSYPSLLQC*LKAPVLSDSSC*K(S) |
| 2457.2005 | [126-149] | (K)SSGSSYPSLLQCLKAPVLSDSSCK(S) |
| 2470.0512 | [160-184] | (N)MIC*VGFLEGGKDSC*QGDSGGPVVCN(G) |
| 2472.0668 | [160-184] | (N)MICVGFLEGGKDSCQGDSGGPVVCN(G) |
| 2483.2161 | [126-149] | (K)SSGSSYPSLLQC*LK**APVLSDSSC*K(S) |
| 2485.2317 | [126-149] | (K)SSGSSYPSLLQCLK**APVLSDSSCK(S) |
| 2486.0461 | [160-184] | (N)M(O)IC*VGFLEGGKDSC*QGDSGGPVVCN(G) |
| 2488.0617 | [160-184] | (N)M(O)ICVGFLEGGKDSCQGDSGGPVVCN(G) |
| 2536.1502 | [20-43] | (N)SGSHFC*GGSLINSQWVVSAAHC*YK(S) |
| 2538.1658 | [20-43] | (N)SGSHFCGGSLINSQWVVSAAHCYK(S) |
| 2624.3295 | [201-223] | (K)NKPGVYTKVCNYVNWIQQTIAAN(<) |
| 2634.3561 | [76-99] | (N)FNGNTLDNDIMLIKLSSPATLNSR(V) |
| 2650.3510 | [76-99] | (N)FNGNTLDNDIM(O)LIKLSSPATLNSR(V) |
| 2652.3607 | [201-223] | (K)NKPGVYTK**VCNYVNWIQQTIAAN(<) |
| 2662.3873 | [76-99] | (N)FNGNTLDNDIMLIK*LSSPATLNSR(V) |
| 2678.3822 | [76-99] | (N)FNGNTLDNDIM(O)LIK*LSSPATLNSR(V) |
| 2707.4168 | [46-69] | (R)IQVRLGEHNIDVLEGNEQFINAAK(I) |
| 2807.3145 | [20-45] | (N)SGSHFC*GGSLINSQWVVSAAHC*YK**SR(I) |
| 2914.5062 | [50-75] | (R)LGEHNIDVLEGNEQFINAAK**IITHPN(F) |
| 3013.3243 | [171-200] | (K)DSCQGDSGGPVVCNGQLQGIVSWGYGCAQK(N) |
| 3094.6246 | [70-97] | (K)IITHPNFNGNTLDNDIMLIK**LSSPATLN(S) |
| 3110.6195 | [70-97] | (K)IITHPNFNGNTLDNDIM(O)LIK*LSSPATLN(S) |
| 3143.4852 | [140-170] | (K)APVLSDSSC*KSSYPGQITGNMIC*VGFLEGGK(D) |
| 3145.5008 | [140-170] | (K)APVLSDSSCKSSYPGQITGNMICVGFLEGGK(D) |
| 3159.4801 | [140-170] | (K)APVLSDSSC*KSSYPGQITGNM(O)IC*VGFLEGGK(D) |
| 3161.4957 | [140-170] | (K)APVLSDSSCKSSYPGQITGNM(O)ICVGFLEGGK(D) |
| 3171.5164 | [140-170] | (K)APVLSDSSC*K**SSYPGQITGNMIC*VGFLEGGK(D) |
| 3173.5320 | [140-170] | (K)APVLSDSSCK**SSYPGQITGNMICVGFLEGGK(D) |
| 3187.5113 | [140-170] | (K)APVLSDSSC*K**SSYPGQITGNM(O)IC*VGFLEGGK(D) |
| 3189.5269 | [140-170] | (K)APVLSDSSCK**SSYPGQITGNM(O)ICVGFLEGGK(D) |
| 3217.4968 | [108-139] | (R)SC*AAAGTEC*LISGWGNTKSSGSSYPSLLQCLK(A) |
| 3219.5125 | [108-139] | (R)SCAAAGTECLISGWGNTKSSGSSYPSLLQCLK(A) |
| 3245.5281 | [108-139] | (R)SC*AAAGTEC*LISGWGNTK**SSGSSYPSLLQCLK(A) |
| 3247.5437 | [108-139] | (R)SCAAAGTECLISGWGNTK**SSGSSYPSLLQCLK(A) |
| 3309.7265 | [70-99] | (K)IITHPNFNGNTLDNDIMLIKLSSPATLNSR(V) |
| 3325.7214 | [70-99] | (K)IITHPNFNGNTLDNDIM(O)LIKLSSPATLNSR(V) |
| 3337.7577 | [70-99] | (K)IITHPNFNGNTLDNDIMLIK**LSSPATLNSR(V) |
| 3353.7526 | [70-79] | (K)IITHPNFNGNTLDNDIM(O)LIK*LSSPATLNSR(V) |
| 3900.8108 | [171-208] | (K)DSCQGDSGGPVVCNGQLQGIVSWGYGCAQKNKPGVYTK(V) |
| 3928.842 | [171-208] | (K)DSCQGDSGGPVVCNGQLQGIVSWGYGCAQK**NKPGVYTK(V) |
| 4475.1011 | [1-43] | (-)IVGGYTCAANSVPYQVSLNSGSHFCGGSLINSQWVVSAAHCYK(S) |
| 4475.2669 | [50-89] | (R)LGEHNIDVLEGNEQFINAAKIITHPNFNGNTLDNDIMLIK(L) |
| 4491.2618 | [50-89] | (R)LGEHNIDVLEGNEQFINAAKIITHPNFNGNTLDNDIM(O)LIK(L) |
| 4503.2981 | [50-89] | (R)LGEHNIDVLEGNEQFINAAK**IITHPNFNGNTLDNDIMLIK(L) |
| 4519.293 | [50-89] | (R)LGEHNIDVLEGNEQFINAAK**IITHPNFNGNTLDNDIM(O)LIK(L) |
| 4718.2343 | [1-45] | (-)IVGGYTCAANSVPYQVSLNSGSHFCGGSLINSQWVVSAAHCYKSR(I) |
| 4746.2655 | [1-45] | (-)IVGGYTCAANSVPYQVSLNSGSHFCGGSLINSQWVVSAAHCYK**SR(I) |
| 5152.3372 | [150-200] | (K)SSYPGQITGNMICVGFLEGGKDSCQGDSGGPVVCNGQLQGIVSWGYGCAQK(N) |
| 5168.3321 | [150-200] | (K)SSYPGQITGNM(O)ICVGFLEGGKDSCQGDSGGPVVCNGQLQGIVSWGYGCAQK(N) |
| 5180.3684 | [150-200] | (K)SSYPGQITGNMICVGFLEGGK*DSCQGDSGGPVVCNGQLQGIVSWGYGCAQK(N) |
| 5196.3633 | [150-200] | (K)SSYPGQITGNM(O)ICVGFLEGGK*DSCQGDSGGPVVCNGQLQGIVSWGYGCAQK(N) |
| 5557.8751 | [50-99] | (R)LGEHNIDVLEGNEQFINAAK**IITHPNFNGNTLDNDIMLIK**LSSPATLNSR(V) |
| 5573.87 | [50-99] | (R)LGEHNIDVLEGNEQFINAAK**IITHPNFNGNTLDNDIM(O)LIK**LSSPATLNSR(V) |
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